Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines.
Arch Virol
; 159(8): 2103-7, 2014 Aug.
Article
in En
| MEDLINE
| ID: mdl-24599565
ABSTRACT
Analysis of complete genome sequences of three Slovak isolates of grapevine Pinot gris virus (GPGV) showed their low heterogeneity (reaching 1.7 %) and a close relationship to the Italian NC_015782 isolate (4.2-4.5 % divergence). Comparison of Slovak and Italian isolates revealed an unusual accumulation of 21 indel mutations in ORF1, resulting in a localized high divergence in the encoded amino acid sequences. An elevated divergence in the 5' extremity of the GPGV genomes is suggestive of a recombination between Slovak isolates and grapevine berry inner necrosis virus. RT-PCR allowed the frequent detection of closely related GPGV isolates in grapevines from Slovakia and the Czech Republic.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Diseases
/
Genetic Variation
/
Vitis
/
Flexiviridae
Type of study:
Diagnostic_studies
Country/Region as subject:
Europa
Language:
En
Journal:
Arch Virol
Year:
2014
Document type:
Article