Expression of bcl-2 and p53 in induction of esophageal cancer cell apoptosis by ECRG2 in combination with cisplatin.
Asian Pac J Cancer Prev
; 15(3): 1397-401, 2014.
Article
in En
| MEDLINE
| ID: mdl-24606472
ABSTRACT
AIM:
To investigate the mechanisms of induction of apoptosis of esophageal cancer cells by esophageal cancer-related gene 2 (ECRG2) in combination with cisplatin (DDP).METHODS:
Hoechest staining was performed to analyze the effects of single ECRG2 and ECRG2 in combination with DDP on apoptosis of EC9706 cells. The expression levels of p53 and bcl-2 mRNA and protein were determined by RT-PCR and Western blotting, respectively.RESULTS:
The number of apoptotic cells after the treatment with ECRG2 in combination with DDP for 24 hours was more than that after the treatment with single ECRG2. RT-PCR and Western blotting showed that the expression levels of bcl-2 mRNA and protein were both down-regulated, while p53 mRNA and protein were both up-regulated in the cells treated with ECRG2 in combination with DDP compared with those given ECRG2 alone.CONCLUSION:
ECRG2 in combination with DDP can enhance the apoptosis of EC9706 cells, possibly by down-regulating bcl-2 expression and up-regulating p53.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Esophageal Neoplasms
/
Tumor Suppressor Protein p53
/
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
/
Proteinase Inhibitory Proteins, Secretory
Limits:
Humans
Language:
En
Journal:
Asian Pac J Cancer Prev
Journal subject:
NEOPLASIAS
Year:
2014
Document type:
Article