Recent applications of fluorescence correlation spectroscopy in live systems.
FEBS Lett
; 588(19): 3571-84, 2014 Oct 01.
Article
in En
| MEDLINE
| ID: mdl-24726724
ABSTRACT
Fluorescence correlation spectroscopy (FCS) is a widely used technique in biophysics and has helped address many questions in the life sciences. It provides important advantages compared to other fluorescence and biophysical methods. Its single molecule sensitivity allows measuring proteins within biological samples at physiological concentrations without the need of overexpression. It provides quantitative data on concentrations, diffusion coefficients, molecular transport and interactions even in live organisms. And its reliance on simple fluorescence intensity and its fluctuations makes it widely applicable. In this review we focus on applications of FCS in live samples, with an emphasis on work in the last 5 years, in the hope to provide an overview of the present capabilities of FCS to address biologically relevant questions.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spectrometry, Fluorescence
/
Biology
Limits:
Animals
/
Humans
Language:
En
Journal:
FEBS Lett
Year:
2014
Document type:
Article
Affiliation country: