Motor cortex glutathione deficit in ALS measured in vivo with the J-editing technique.
Neurosci Lett
; 570: 102-7, 2014 Jun 06.
Article
in En
| MEDLINE
| ID: mdl-24769125
ABSTRACT
This study compared in vivo levels of the antioxidant glutathione (GSH) in the motor cortex of 11 ALS patients with those in 11 age-matched healthy volunteers (HV). Using the standard J-edited spin-echo difference MRS technique, GSH spectra were recorded on a 3.0 T GE MR system from a single precentral gyrus voxel. GSH levels expressed as ratios to the unsuppressed voxel tissue water (W) were 31% lower in ALS patients than in HV (p=.005), and 36% lower in ALS than in HV (p=.02) when expressed as ratios to the total creatine peak (tCr), supporting a role for oxidative stress in ALS. Levels of the putative neuronal marker N-acetylaspartate (NAA) relative to W did not differ between ALS and HV (p=.26), but were lower by 9% in ALS than in HV (p=.013) when expressed as ratios relative to tCr. This discrepancy is attributed to small but opposite changes in NAA and tCr in ALS that, as a ratio, resulted in a statistically significant group difference, further suggesting caution in using tCr as an internal reference under pathological conditions.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Glutathione
/
Amyotrophic Lateral Sclerosis
/
Motor Cortex
Type of study:
Observational_studies
/
Risk_factors_studies
Limits:
Aged
/
Female
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Humans
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Male
/
Middle aged
Language:
En
Journal:
Neurosci Lett
Year:
2014
Document type:
Article
Affiliation country: