Effect of sterilization methods on cell binding activity of surface-immobilized fibronectin.
Biomed Environ Sci
; 27(5): 391-5, 2014 May.
Article
in En
| MEDLINE
| ID: mdl-24827722
ABSTRACT
The effect of sterilization methods on biological activity of fibronectin on the surface of biomaterials was elaborated in the present study. Sterile protein- modified biomaterials were fabricated by microfilter filtration and UV irradiation, respectively. UV irradiation altered the conformation of surface- adsorbed fibronectin and further affected the attachment, morphology and biological function of endothelial cells. However, microfilter filtration did not to change the normal conformation of fibronectin, or the proliferation and biological function of endothelial cells, indicating that microfilter filtration sterilization is the most suitable method for protein-substrate.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Prostheses and Implants
/
Sterilization
/
Fibronectins
Type of study:
Evaluation_studies
Language:
En
Journal:
Biomed Environ Sci
Journal subject:
SAUDE AMBIENTAL
Year:
2014
Document type:
Article
Affiliation country: