Methylene blue attenuates ischemia--reperfusion injury in lung transplantation.
J Surg Res
; 192(2): 635-41, 2014 Dec.
Article
in En
| MEDLINE
| ID: mdl-25151469
ABSTRACT
BACKGROUND:
Ischemia-reperfusion injury (IRI) is one of the principal obstacles for the lung transplantation (LTx) success. Several strategies have been adopted to minimize the effects of IRI in lungs, including ex vivo conditioning of the grafts and the use of antioxidant drugs, such as methylene blue (MB). We hypothesized that MB could minimize the effects of IRI in a LTx rodent model.METHODS:
Forty rats were divided into four groups (n = 10) according to treatment (saline solution or MB) and graft cold ischemic time (3 or 6 h). All animals underwent unilateral LTx. Recipients received 2 mL of saline or MB intraperitoneally before transplantation. After 2 h of reperfusion, arterial blood and exhaled nitric oxide samples were collected and bronchoalveolar lavage performed. Then animals were euthanized, and histopathology analysis as well as cell counts and cytokine levels measurements in bronchoalveolar lavage fluid were performed.RESULTS:
There was a significant decrease in exhaled nitric oxide, neutrophils, interleukin-6, and tumor necrosis factor-α in MB-treated animals. PaO2 and uric acid levels were higher in MB group.CONCLUSIONS:
MB was able in attenuating IRI in this LTx model.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Reperfusion Injury
/
Lung Transplantation
/
Methylene Blue
/
Antioxidants
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J Surg Res
Year:
2014
Document type:
Article