Red-mediated recombineering of Salmonella enterica genomes.
Methods Mol Biol
; 1225: 63-79, 2015.
Article
in En
| MEDLINE
| ID: mdl-25253248
ABSTRACT
The mutagenesis of enterobacterial genomes using phage λ Red recombinase functions is a rapid and versatile experimental tool. In addition to the rapid generation of deletions in the genome of Salmonella enterica, variations of the method allow site-directed mutagenesis, generation of reporter fusions, generation of chimeric genes, or transplantation of regulatory elements directly in the chromosome. We describe the application of these approaches with focus on practical aspects and critical steps.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Genetic Engineering
/
Genome, Bacterial
/
Bacteriophage lambda
/
Salmonella enterica
/
Recombinases
/
Homologous Recombination
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2015
Document type:
Article
Affiliation country: