Development of the fetal bone marrow niche and regulation of HSC quiescence and homing ability by emerging osteolineage cells.
Cell Rep
; 9(2): 581-90, 2014 Oct 23.
Article
in En
| MEDLINE
| ID: mdl-25310984
ABSTRACT
Hematopoietic stem cells (HSCs) reside within a specialized niche where interactions with vasculature, osteoblasts, and stromal components regulate their self-renewal and differentiation. Little is known about bone marrow niche formation or the role of its cellular components in HSC development; therefore, we established the timing of murine fetal long bone vascularization and ossification relative to the onset of HSC activity. Adult-repopulating HSCs emerged at embryonic day 16.5 (E16.5), coincident with marrow vascularization, and were contained within the c-Kit(+)Sca-1(+)Lin(-) (KSL) population. We used Osterix-null (Osx(-/-)) mice that form vascularized marrow but lack osteolineage cells to dissect the role(s) of these cellular components in HSC development. Osx(-/-) fetal bone marrow cells formed multilineage colonies in vitro but were hyperproliferative and failed to home to and/or engraft transplant recipients. Thus, in developing bone marrow, the vasculature can sustain multilineage progenitors, but interactions with osteolineage cells are needed to regulate long-term HSC proliferation and potential.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteogenesis
/
Bone Marrow
/
Hematopoietic Stem Cells
/
Embryonic Stem Cells
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Stem Cell Niche
Limits:
Animals
Language:
En
Journal:
Cell Rep
Year:
2014
Document type:
Article