Effects of gender, cytokine gene polymorphisms and environmental factors on inflammatory responses.
Innate Immun
; 21(5): 523-30, 2015 Jul.
Article
in En
| MEDLINE
| ID: mdl-25432967
ABSTRACT
Previous studies have indicated that cytokine gene polymorphisms of Indigenous Australians were predominantly associated with strong pro-inflammatory responses. We tested the hypothesis that cells of donors with genetic profiles of inflammatory cytokine single nucleotide polymorphisms (SNPs) similar to Indigenous Australians produce higher pro-inflammatory responses. PBMCs from 14 donors with genetic profiles for a high risk of strong pro-inflammatory responses and 14 with low-risk profiles were stimulated with endotoxin and effects of gender, IFN-γ, cigarette smoke extract (CSE) and testosterone on cytokine responses analysed. Cytokines were calculated from standard curves (Luminex 2.3 software). No significant differences were associated with SNP profile alone. Lower pro-inflammatory responses were observed for cells from males with low- or high-risk profiles. For cells from females with high-risk profiles, anti-inflammatory IL-10 responses were significantly reduced. There was no effect of testosterone levels on responses from males. For females, results from IFN-γ-treated cells showed positive correlations between testosterone levels and IL-1ß responses to endotoxin for both risk groups and TNF-α for the high-risk group. If interactions observed among CSE, IFN-γ, genetic background and testosterone reflect those in vivo, these might contribute to increased incidences of hospitalisations for infectious diseases among Indigenous women.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polymorphism, Genetic
/
Tobacco Smoke Pollution
/
Cytokines
/
Environment
/
Inflammation
Type of study:
Etiology_studies
/
Risk_factors_studies
Aspects:
Determinantes_sociais_saude
Limits:
Adult
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Female
/
Humans
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Male
/
Middle aged
Language:
En
Journal:
Innate Immun
Journal subject:
ALERGIA E IMUNOLOGIA
/
BACTERIOLOGIA
Year:
2015
Document type:
Article
Affiliation country: