RNA sequencing of mouse sinoatrial node reveals an upstream regulatory role for Islet-1 in cardiac pacemaker cells.
Circ Res
; 116(5): 797-803, 2015 Feb 27.
Article
in En
| MEDLINE
| ID: mdl-25623957
ABSTRACT
RATIONALE Treatment of sinus node disease with regenerative or cell-based therapies will require a detailed understanding of gene regulatory networks in cardiac pacemaker cells (PCs). OBJECTIVE:
To characterize the transcriptome of PCs using RNA sequencing and to identify transcriptional networks responsible for PC gene expression. METHODS ANDRESULTS:
We used laser capture microdissection on a sinus node reporter mouse line to isolate RNA from PCs for RNA sequencing. Differential expression and network analysis identified novel sinoatrial node-enriched genes and predicted that the transcription factor Islet-1 is active in developing PCs. RNA sequencing on sinoatrial node tissue lacking Islet-1 established that Islet-1 is an important transcriptional regulator within the developing sinoatrial node.CONCLUSIONS:
(1) The PC transcriptome diverges sharply from other cardiomyocytes; (2) Islet-1 is a positive transcriptional regulator of the PC gene expression program.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sinoatrial Node
/
Transcription Factors
/
RNA, Messenger
/
Gene Expression Regulation, Developmental
/
Myocytes, Cardiac
/
LIM-Homeodomain Proteins
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Circ Res
Year:
2015
Document type:
Article