Differentiation between fresh and frozen-thawed sea bass (Dicentrarchus labrax) fillets using two-dimensional gel electrophoresis.
Food Chem
; 176: 294-301, 2015 Jun 01.
Article
in En
| MEDLINE
| ID: mdl-25624236
ABSTRACT
This study aimed to identify a protein marker that can differentiate between fresh skinless and frozen-thawed sea bass (Dicentrarchus labrax) fillets using the two-dimensional polyacrylamide gel electrophoresis (2-DE) technique. Distinct gel patterns, due to proteins with low molecular weight and low isoelectric points, distinguished fresh fillets from frozen-thawed ones. Frozen-thawed fillets showed two specific protein spots as early as the first day of the study. However, these spots were not observed in fresh fillets until at least 13days of storage between 0 and 4°C, fillets were judged, beyond this period, fish were unfit for human consumption as revealed by complementary studies on fish spoilage indicators namely total volatile basic nitrogen and biogenic amines. Mass spectrometry identified the specific proteins as parvalbumin isoforms. Parvalbumins may thus be useful markers of differentiation between fresh and frozen-thawed sea bass fillets.
Key words
2D-electrophoresis; 3-[(3-Cholamidopropyl)dimethylammonium]-1-propanesulfonate (PubChem CID: 107670); Acetonitrile (PubChem CID: 6342); Biogenic amines; Diaminopropane (PubChem CID: 6567); Dithiothreitol (PubChem CID: 19001); Ethanol (PubChem CID: 702); Formic acid (PubChem CID: 284); Fresh; Frozenthawed; Iodoacetamide (PubChem CID: 3727); Perchloric acid (PubChem CID: 24247); Sea bass (Dicentrarchus labrax); Tris(hydroxymethyl)aminomethane hydrochloride (PubChem CID: 93573); Urea (PubChem CID: 1176)
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bass
/
Electrophoresis, Gel, Two-Dimensional
/
Seafood
Limits:
Animals
/
Humans
Language:
En
Journal:
Food Chem
Year:
2015
Document type:
Article