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Target specificity of the CRISPR-Cas9 system.
Wu, Xuebing; Kriz, Andrea J; Sharp, Phillip A.
Affiliation
  • Wu X; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA ; Computational and Systems Biology Graduate Program, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Kriz AJ; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Sharp PA; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA ; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Quant Biol ; 2(2): 59-70, 2014 Jun.
Article in En | MEDLINE | ID: mdl-25722925
ABSTRACT
The CRISPR-Cas9 system, naturally a defense mechanism in prokaryotes, has been repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome editing and transcriptome modulation, and has shown great promise in correcting mutations in human genetic diseases. Off-target effects are a critical issue for all of these applications. Here we review the current status on the target specificity of the CRISPR-Cas9 system.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Quant Biol Year: 2014 Document type: Article Affiliation country:
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Quant Biol Year: 2014 Document type: Article Affiliation country: