Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli.
Bioresour Technol
; 185: 56-61, 2015 Jun.
Article
in En
| MEDLINE
| ID: mdl-25747879
ABSTRACT
In this study, microbial production of succinic acid from macroalgae (i.e., Laminaria japonica) was investigated for the first time. The engineered Escherichia coli BS002 exhibited higher molar yield of succinic acid on mannitol (1.39±0.01mol/mol) than glucose (1.01±0.05mol/mol). After pretreatment and enzymatic hydrolysis, L. japonica hydrolysate was mainly glucose (10.31±0.32g/L) and mannitol (10.12±0.17g/L), which was used as the substrate for succinic acid fermentation with the recombinant BS002. A final 17.44±0.54g/L succinic acid was obtained from the hydrolysate after 72h dual-phase fermentation. The yield was as high as 1.24±0.08mol/mol total sugar, which reached 73% of the maximum theoretical yield. The results demonstrate that macroalgae biomass represents a novelty and economical alternative feedstock for biochemicals production.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Seaweed
/
Succinic Acid
/
Escherichia coli
/
Metabolic Engineering
/
Glucose
/
Mannitol
Language:
En
Journal:
Bioresour Technol
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2015
Document type:
Article