Laser Microdissection of FFPE Tissue Areas and Subsequent Whole Genome Amplification by Ampli1™.
Methods Mol Biol
; 1347: 141-62, 2015.
Article
in En
| MEDLINE
| ID: mdl-26374316
ABSTRACT
Laser microdissection (LMD) and whole genome amplification (WGA) are valuable tools to isolate, purify, and genetically analyze cancer cells from tissue sections. In this chapter, we describe a workflow for microdissecting small regions of interest from cancer tissue, i.e. formalin-fixed paraffin-embedded (FFPE) and cryo-conserved specimens, and subsequent whole genome amplification by a deterministic WGA approach (Ampli1™ WGA).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Genome, Human
/
Nucleic Acid Amplification Techniques
/
Genomics
/
Laser Capture Microdissection
Limits:
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2015
Document type:
Article
Affiliation country: