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Laser Microdissection of FFPE Tissue Areas and Subsequent Whole Genome Amplification by Ampli1™.
Czyz, Zbigniew Tadeusz; Stoecklein, Nikolas H; Polzer, Bernhard.
Affiliation
  • Czyz ZT; Project Group, Personalized Tumor Therapy, Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Josef-Engert-Straße 9, 93053, Regensburg, Germany.
  • Stoecklein NH; Department of Visceral, General, and Pediatric Surgery, University Hospital and Medical Faculty of the Heinrich-Heine University Düsseldorf, Moorenstr. 5, 40225, Düsseldorf, Germany.
  • Polzer B; Project Group, Personalized Tumor Therapy, Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Josef-Engert-Straße 9, 93053, Regensburg, Germany. bernhard.michael.polzer@item.fraunhofer.de.
Methods Mol Biol ; 1347: 141-62, 2015.
Article in En | MEDLINE | ID: mdl-26374316
ABSTRACT
Laser microdissection (LMD) and whole genome amplification (WGA) are valuable tools to isolate, purify, and genetically analyze cancer cells from tissue sections. In this chapter, we describe a workflow for microdissecting small regions of interest from cancer tissue, i.e. formalin-fixed paraffin-embedded (FFPE) and cryo-conserved specimens, and subsequent whole genome amplification by a deterministic WGA approach (Ampli1™ WGA).
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Genome, Human / Nucleic Acid Amplification Techniques / Genomics / Laser Capture Microdissection Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2015 Document type: Article Affiliation country:
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Genome, Human / Nucleic Acid Amplification Techniques / Genomics / Laser Capture Microdissection Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2015 Document type: Article Affiliation country: