Effect of cisplatin on proteasome activity.
J Inorg Biochem
; 153: 253-258, 2015 Dec.
Article
in En
| MEDLINE
| ID: mdl-26387966
ABSTRACT
Cisplatin is a widely used chemotherapy drug which exerts cytotoxic activity by affecting both nuclear and cytosolic pathways. Herewith, we report, for the first time, that cisplatin inhibits proteasome activity in vitro. Cisplatin induces a dose dependent inhibition of the three enzymatic activities of proteasome (i.e., the chymotrypsin-like activity, the trypsin-like activity and the caspase-like activity). Moreover, cisplatin administration to neuroblastoma cells brings about a fast loss of proteasome particle activity, which is followed by a de novo synthesis of proteasome. Lastly, we report that the simultaneous administration of lactacystin and cisplatin enhances the cytotoxicity of cisplatin alone. The overall bulk of data opens to an intriguing scenario, concerning the biological effects of cisplatin in the control of cellular life, which goes beyond the well established genotoxic effect.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cysteine Proteinase Inhibitors
/
Cisplatin
/
Proteasome Endopeptidase Complex
/
Proteasome Inhibitors
/
Antineoplastic Agents
Limits:
Humans
Language:
En
Journal:
J Inorg Biochem
Year:
2015
Document type:
Article
Affiliation country: