The insulin-mimetic effect of vanadate is not correlated with insulin receptor tyrosine kinase activity nor phosphorylation in mouse diaphragm in vivo.
Endocrinology
; 124(4): 1918-24, 1989 Apr.
Article
in En
| MEDLINE
| ID: mdl-2647469
ABSTRACT
The in vivo administration of sodium orthovanadate stimulated the incorporation of [14C]glucose into [14C] glycogen, in a dose- and time-dependent manner, in mouse diaphragm. Activation of diaphragm insulin receptor was measured by exogenous tyrosine kinase activity and an antibody that recognizes a conformational change in the receptor beta-subunit upon autophosphorylation. Neither method detected insulin receptor activation by in vivo vanadate administration, suggesting that vanadate's insulin-mimetic effect on mouse diaphragm glycogenesis occurs at a site distal to the insulin receptor.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protein-Tyrosine Kinases
/
Receptor, Insulin
/
Vanadates
/
Insulin
/
Muscles
Limits:
Animals
Language:
En
Journal:
Endocrinology
Year:
1989
Document type:
Article