Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy.
Nanoscale
; 8(25): 12626-38, 2016 Jul 07.
Article
in En
| MEDLINE
| ID: mdl-26733445
ABSTRACT
In the present work we have devised the synthesis of a novel promising carbon nanotube carrier for the targeted delivery of radioactivity, through a combination of endohedral and exohedral functionalization. Steam-purified single-walled carbon nanotubes (SWCNTs) have been initially filled with radioactive analogues (i.e. metal halides) and sealed by high temperature treatment, affording closed-ended CNTs with the filling material confined in the inner cavity. The external functionalization of these filled CNTs was then achieved by nitrene cycloaddition and followed by the derivatization with a monoclonal antibody (Cetuximab) targeting the epidermal growth factor receptor (EGFR), overexpressed by several cancer cells. The targeting efficiency of the so-obtained conjugate was evaluated by immunostaining with a secondary antibody and by incubation of the CNTs with EGFR positive cells (U87-EGFR+), followed by flow cytometry, confocal microscopy or elemental analyses. We demonstrated that our filled and functionalized CNTs can internalize more efficiently in EGFR positive cancer cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nanotubes, Carbon
/
Cetuximab
/
Metals
/
Neoplasms
Limits:
Animals
/
Humans
Language:
En
Journal:
Nanoscale
Year:
2016
Document type:
Article
Affiliation country: