Research Techniques Made Simple: Techniques to Assess Cell Proliferation.
J Invest Dermatol
; 136(1): e1-e7, 2016 Jan.
Article
in En
| MEDLINE
| ID: mdl-26763463
ABSTRACT
Cell proliferation is commonly assayed in the laboratory for research purposes, but is increasingly used clinically to gauge tumor aggressiveness and potentially guide care. Therefore, both researchers and clinicians should have a basic understanding of techniques used to assess cell proliferation. Multiple cell proliferation assays exist, and the choice of method depends on the laboratory resources available, the types of cells/tissues to be studied, and the specific experimental goals. In this article, we identify four overarching categories of cell proliferation assays that signify various stages of the cell cycle nucleoside-analog incorporation, cell cycle-associated protein detection, use of cytoplasmic proliferation dyes, and indirect measures of cell proliferation. Each method has strengths and limitations that should guide the dermatology investigator's choice of assay.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Cycle
/
Cell Proliferation
/
Cell Migration Assays
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
J Invest Dermatol
Year:
2016
Document type:
Article
Affiliation country: