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Cloning and characterization of 5-enopyruvylshikimate-3-phosphate synthase from Pantoea sp.
Liu, F; Cao, Y P.
Affiliation
  • Liu F; Plant Science Department, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, China.
  • Cao YP; Plant Science Department, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, China.
Genet Mol Res ; 14(4): 19233-41, 2015 Dec 29.
Article in En | MEDLINE | ID: mdl-26782576
ABSTRACT
The shikimate pathway enzyme 5-enopyruvylshikimate-3-phosphate synthase (EPSPS) is the target of the broad spectrum herbicide glyphosate. A novel aroA gene encoding an EPSPS from Pantoea sp was identified and subcloned into the pET-28a vector to construct the recombinant pET-AroAPantoea sp plasmid. Amino acid sequence analysis indicated that AroAPantoea sp is a class I AroA enzyme. When expressed in Escherichia coli, it conveyed high tolerance to glyphosate. AroAPantoea sp may be used to generate transgenic glyphosate-tolerant plants.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Soil Microbiology / Bacterial Proteins / Cloning, Molecular / Pantoea / 3-Phosphoshikimate 1-Carboxyvinyltransferase Language: En Journal: Genet Mol Res Journal subject: BIOLOGIA MOLECULAR / GENETICA Year: 2015 Document type: Article Affiliation country:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Soil Microbiology / Bacterial Proteins / Cloning, Molecular / Pantoea / 3-Phosphoshikimate 1-Carboxyvinyltransferase Language: En Journal: Genet Mol Res Journal subject: BIOLOGIA MOLECULAR / GENETICA Year: 2015 Document type: Article Affiliation country: