Cloning and characterization of 5-enopyruvylshikimate-3-phosphate synthase from Pantoea sp.
Genet Mol Res
; 14(4): 19233-41, 2015 Dec 29.
Article
in En
| MEDLINE
| ID: mdl-26782576
ABSTRACT
The shikimate pathway enzyme 5-enopyruvylshikimate-3-phosphate synthase (EPSPS) is the target of the broad spectrum herbicide glyphosate. A novel aroA gene encoding an EPSPS from Pantoea sp was identified and subcloned into the pET-28a vector to construct the recombinant pET-AroAPantoea sp plasmid. Amino acid sequence analysis indicated that AroAPantoea sp is a class I AroA enzyme. When expressed in Escherichia coli, it conveyed high tolerance to glyphosate. AroAPantoea sp may be used to generate transgenic glyphosate-tolerant plants.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Soil Microbiology
/
Bacterial Proteins
/
Cloning, Molecular
/
Pantoea
/
3-Phosphoshikimate 1-Carboxyvinyltransferase
Language:
En
Journal:
Genet Mol Res
Journal subject:
BIOLOGIA MOLECULAR
/
GENETICA
Year:
2015
Document type:
Article
Affiliation country: