Cloning and expression of the cDNA for bovine granulocyte-macrophage colony-stimulating factor.
Vet Immunol Immunopathol
; 21(3-4): 261-78, 1989 Jul.
Article
in En
| MEDLINE
| ID: mdl-2678728
ABSTRACT
A sequence encoding bovine granulocyte-macrophage colony-stimulating factor (GM-CSF) has been identified from a concanavalin A-stimulated bovine lymphocyte cDNA library. This sequence was isolated by hybridization with synthetic oligonucleotide probes based upon the human GM-CSF sequence. This bovine cDNA was engineered for expression and secretion of activity into the periplasmic space of E. coli. Periplasmic extracts contain a 14,500-dalton protein and stimulate colony formation of bovine bone marrow progenitor cells. The predicted protein is 70% homologous with human GM-CSF and 55% homologous with murine GM-CSF. Numerous structural features are conserved among these three proteins, such as location of cysteine residues, glycosylation sites, and overall change. The biological activity of bovine GM-CSF is species specific, since recombinant preparations do not cause proliferation of human or murine bone marrow cells. Similarly, murine GM-CSF does not exhibit activity on cells of bovine or human origin. However, human GM-CSF does stimulate colony formation of bovine bone marrow cells, although the specific activity appears reduced when compared to assays on human cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA
/
Cattle
/
Colony-Stimulating Factors
/
Growth Substances
/
Cloning, Molecular
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Vet Immunol Immunopathol
Year:
1989
Document type:
Article