Sleep-promoting effects of a GABA/5-HTP mixture: Behavioral changes and neuromodulation in an invertebrate model.
Life Sci
; 150: 42-9, 2016 Apr 01.
Article
in En
| MEDLINE
| ID: mdl-26921634
ABSTRACT
AIMS:
This study was to investigate the sleep promoting effects of combined γ-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP), by examining neuronal processes governing mRNA level alterations, as well as assessing neuromodulator concentrations, in a fruit fly model. MAINMETHODS:
Behavioral assays were applied to investigate subjective nighttime activity, sleep episodes, and total duration of subjective nighttime sleep of two amino acids and GABA/5-HTP mixture with caffeine treated flies. Also, real-time PCR and HPLC analysis were applied to analyze the signaling pathway. KEYFINDINGS:
Subjective nighttime activity and sleep patterns of individual flies significantly decreased with 1% GABA treatment in conjunction with 0.1% 5-HTP treatment (p<0.001). Furthermore, GABA/5-HTP mixture resulted in significant differences between groups related to sleep patterns (40%, p<0.017) and significantly induced subjective nighttime sleep in the awake model (p<0.003). These results related to transcript levels of the GABAB receptor (GABAB-R1) and serotonin receptor (5-HT1A), compared to the control group. In addition, GABA/5-HTP mixture significantly increased GABA levels 1h and 12h following treatment (2.1 fold and 1.2 fold higher than the control, respectively) and also increased 5-HTP levels (0 h 1.01 µg/protein, 12h 3.45 µg/protein).SIGNIFICANCE:
In this regard, we successfully demonstrated that using a GABA/5-HTP mixture modulates subjective nighttime activity, sleep episodes, and total duration of subjective nighttime sleep to a greater extent than single administration of each amino acid, and that this modulation occurs via GABAergic and serotonergic signaling.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sleep
/
Behavior, Animal
/
5-Hydroxytryptophan
/
Drosophila melanogaster
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Gamma-Aminobutyric Acid
/
Hypnotics and Sedatives
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Life Sci
Year:
2016
Document type:
Article