Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae.
Sci Rep
; 6: 22252, 2016 Mar 07.
Article
in En
| MEDLINE
| ID: mdl-26947136
ABSTRACT
Of the three dominant marine microalgal groups, dinoflagellates and diatoms can undergo genetic transformation; however, no transformation method has been established for haptophytes to date. Here, we report the first stable genetic transformation of a coccolithophore, Pleurochrysis carterae, by means of polyethylene glycol (PEG)-mediated transfer of a bacterial hygromycin B-resistance gene. Together with the novel transient green fluorescent protein (GFP) expression system, this approach should facilitate further molecular-based research in this phylum.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transformation, Genetic
/
Haptophyta
/
Genetics, Microbial
/
Molecular Biology
Language:
En
Journal:
Sci Rep
Year:
2016
Document type:
Article
Affiliation country: