Ease-of-use protocol for the rapid detection of third-generation cephalosporin resistance in Enterobacteriaceae isolated from blood cultures using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry.
J Hosp Infect
; 93(2): 206-10, 2016 Jun.
Article
in En
| MEDLINE
| ID: mdl-27105753
ABSTRACT
An ease-of-use protocol for the identification of resistance against third-generation cephalosporins in Enterobacteriaceae isolated from blood culture bottles was evaluated using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. A cefotaxime hydrolysis assay from chocolate agar subcultures using antibiotic discs and without inoculum standardization was developed for routine work flow, with minimal hands-on time. This assay showed good performance in distinguishing between cefotaxime-susceptible and cefotaxime-resistant strains, with excellent results for Escherichia coli (sensitivity 94.7%, specificity 100%). However, cefotaxime resistance was not detected reliably in Enterobacteriaceae expressing AmpC genes or carbapenemase-producing Klebsiella pneumoniae.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Microbial Sensitivity Tests
/
Cephalosporin Resistance
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Enterobacteriaceae
/
Blood Culture
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Language:
En
Journal:
J Hosp Infect
Year:
2016
Document type:
Article