Paeoniflorin Promotes Angiogenesis in A Vascular Insufficiency Model of Zebrafish in vivo and in Human Umbilical Vein Endothelial Cells in vitro.
Chin J Integr Med
; 24(7): 494-501, 2018 Jul.
Article
in En
| MEDLINE
| ID: mdl-27286711
ABSTRACT
OBJECTIVE:
To investigate the pro-angiogenic effects of paeoniflorin (PF) in a vascular insufficiency model of zebrafish and in human umbilical vein endothelial cells (HUVECs).METHODS:
In vivo, the pro-angiogenic effects of PF were tested in a vascular insufficiency model in the Tg(fli-1EGFP)y1 transgenic zebrafish. The 24 h post fertilization (hpf) embryos were pretreated with vascular endothelial growth factor (VEGF) receptor tyrosine kinase inhibitor II (VRI) for 3 h to establish the vascular insufficiency model and then post-treated with PF for 24 h. The formation of intersegmental vessels (ISVs) was observed with a fluorescence microscope. The mRNA expression of fms-like tyrosine kinase-1 (flt-1), kinase insert domain receptor (kdr), kinase insert domain receptor like (kdrl) and von Willebrand factor (vWF) were analyzed by real-time polymerase chain reaction (PCR). In vitro, the pro-angiogenic effects of PF were observed in HUVECs in which cell proliferation, migration and tube formation were assessed.RESULTS:
PF (6.25-100 µmol/L) could rescue VRI-induced blood vessel loss in zebrafish and PF (25-100 µmol/L), thereby restoring the mRNA expressions of flt-1, kdr, kdrl and vWF, which were down-regulated by VRI treatment. In addition, PF (0.001-0.03 µmol/L) could promote the proliferation of HUVECs while PF stimulated HUVECs migration at 1.0-10 µmol/L and tube formation at 0.3 µmol/L.CONCLUSION:
PF could promote angiogenesis in a vascular insufficiency model of zebrafish in vivo and in HUVECs in vitro.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vascular Diseases
/
Neovascularization, Physiologic
/
Monoterpenes
/
Angiogenesis Inducing Agents
/
Human Umbilical Vein Endothelial Cells
/
Glucosides
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Chin J Integr Med
Journal subject:
TERAPIAS COMPLEMENTARES
Year:
2018
Document type:
Article
Affiliation country: