Highly efficient enzyme encapsulation in a protein nanocage: towards enzyme catalysis in a cellular nanocompartment mimic.
Nanoscale
; 8(30): 14467-72, 2016 Aug 14.
Article
in En
| MEDLINE
| ID: mdl-27407020
ABSTRACT
The study of enzyme behavior in small nanocompartments is crucial for the understanding of biocatalytic processes in the cellular environment. We have developed an enzymatic conjugation strategy to attach a model enzyme to the interior of a cowpea chlorotic mottle virus capsid. It is shown that with this methodology high encapsulation efficiencies can be achieved. Additionally, we demonstrate that the encapsulation does not affect the enzyme performance in terms of a decreased activity or a hampered substrate diffusion. Finally, it is shown that the encapsulated enzymes are protected against proteases. We believe that our strategy can be used to study enzyme kinetics in an environment that approaches physiological conditions.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Fungal Proteins
/
Capsid
/
Biomimetic Materials
/
Lipase
Language:
En
Journal:
Nanoscale
Year:
2016
Document type:
Article
Affiliation country: