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Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography.
Nogly, Przemyslaw; Panneels, Valerie; Nelson, Garrett; Gati, Cornelius; Kimura, Tetsunari; Milne, Christopher; Milathianaki, Despina; Kubo, Minoru; Wu, Wenting; Conrad, Chelsie; Coe, Jesse; Bean, Richard; Zhao, Yun; Båth, Petra; Dods, Robert; Harimoorthy, Rajiv; Beyerlein, Kenneth R; Rheinberger, Jan; James, Daniel; DePonte, Daniel; Li, Chufeng; Sala, Leonardo; Williams, Garth J; Hunter, Mark S; Koglin, Jason E; Berntsen, Peter; Nango, Eriko; Iwata, So; Chapman, Henry N; Fromme, Petra; Frank, Matthias; Abela, Rafael; Boutet, Sébastien; Barty, Anton; White, Thomas A; Weierstall, Uwe; Spence, John; Neutze, Richard; Schertler, Gebhard; Standfuss, Jörg.
Affiliation
  • Nogly P; Laboratory for Biomolecular Research, Paul Scherrer Institute, Villigen 5232, Switzerland.
  • Panneels V; Laboratory for Biomolecular Research, Paul Scherrer Institute, Villigen 5232, Switzerland.
  • Nelson G; Department of Physics, Arizona State University, Tempe, Arizona 85287, USA.
  • Gati C; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607 Hamburg, Germany.
  • Kimura T; Biometal Science Laboratory, RIKEN SPring-8 Center, Hyogo 679-5148, Japan.
  • Milne C; SwissFEL, Paul Scherrer Institute, Villigen 5232, Switzerland.
  • Milathianaki D; Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
  • Kubo M; Biometal Science Laboratory, RIKEN SPring-8 Center, Hyogo 679-5148, Japan.
  • Wu W; PRESTO, JST, Saitama 332-0012, Japan.
  • Conrad C; Laboratory for Biomolecular Research, Paul Scherrer Institute, Villigen 5232, Switzerland.
  • Coe J; Department of Chemistry and Biochemistry, and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona 85287-1604, USA.
  • Bean R; Department of Chemistry and Biochemistry, and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona 85287-1604, USA.
  • Zhao Y; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607 Hamburg, Germany.
  • Båth P; Department of Physics, Arizona State University, Tempe, Arizona 85287, USA.
  • Dods R; Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, SE-40530 Gothenburg, Sweden.
  • Harimoorthy R; Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, SE-40530 Gothenburg, Sweden.
  • Beyerlein KR; Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, SE-40530 Gothenburg, Sweden.
  • Rheinberger J; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607 Hamburg, Germany.
  • James D; Laboratory for Biomolecular Research, Paul Scherrer Institute, Villigen 5232, Switzerland.
  • DePonte D; Laboratory for Biomolecular Research, Paul Scherrer Institute, Villigen 5232, Switzerland.
  • Li C; Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
  • Sala L; Department of Physics, Arizona State University, Tempe, Arizona 85287, USA.
  • Williams GJ; SwissFEL, Paul Scherrer Institute, Villigen 5232, Switzerland.
  • Hunter MS; Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
  • Koglin JE; Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
  • Berntsen P; Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
  • Nango E; Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, SE-40530 Gothenburg, Sweden.
  • Iwata S; SACLA Science Research Group, RIKEN/SPring-8 Center, Hyogo 679-5148, Japan.
  • Chapman HN; SACLA Science Research Group, RIKEN/SPring-8 Center, Hyogo 679-5148, Japan.
  • Fromme P; Department of Cell Biology, Kyoto University, Kyoto 606-8501, Japan.
  • Frank M; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607 Hamburg, Germany.
  • Abela R; Department of Physics, University of Hamburg, 22761 Hamburg, Germany.
  • Boutet S; Centre for Ultrafast Imaging, University of Hamburg, 22761 Hamburg, Germany.
  • Barty A; Department of Chemistry and Biochemistry, and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, Tempe, Arizona 85287-1604, USA.
  • White TA; Lawrence Livermore National Laboratory, Livermore 94550, USA.
  • Weierstall U; SwissFEL, Paul Scherrer Institute, Villigen 5232, Switzerland.
  • Spence J; Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
  • Neutze R; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607 Hamburg, Germany.
  • Schertler G; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607 Hamburg, Germany.
  • Standfuss J; Department of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Nat Commun ; 7: 12314, 2016 08 22.
Article in En | MEDLINE | ID: mdl-27545823
ABSTRACT
Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacteriorhodopsins / Crystallography, X-Ray / Lasers / Lipids Aspects: Implementation_research Language: En Journal: Nat Commun Journal subject: BIOLOGIA / CIENCIA Year: 2016 Document type: Article Affiliation country:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacteriorhodopsins / Crystallography, X-Ray / Lasers / Lipids Aspects: Implementation_research Language: En Journal: Nat Commun Journal subject: BIOLOGIA / CIENCIA Year: 2016 Document type: Article Affiliation country: