Isolation of Actin and Actin-Binding Proteins.
Methods Mol Biol
; 1511: 291-299, 2017.
Article
in En
| MEDLINE
| ID: mdl-27730620
ABSTRACT
Actin-binding proteins mediate and regulate the dynamics of actin and the organization of highly ordered structures of F-actin. Villin is generally expressed in plant cells and is associated with G-actin or F-actin dependent on Ca2+ concentrations. Using a DNase I affinity column chromatography approach, the villin and the G-actin can be isolated from plant material. An outline of this method including the preparation of crude protein extract from plant material, its application on the affinity column, and the successive elution of villin with a solution containing EGTA and then of G-actin with denatured reagents is presented.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Proteins
/
Chromatography, Affinity
/
Actins
/
Lilium
/
Microfilament Proteins
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2017
Document type:
Article
Affiliation country: