Gelatin Porous Scaffolds as Delivery Systems of Calcium Alendronate.
Macromol Biosci
; 17(3)2017 03.
Article
in En
| MEDLINE
| ID: mdl-27748576
ABSTRACT
The systemic administration of bisphosphonates (BPs) for the treatment of metabolic diseases characterized by abnormal bone loss suffers from several adverse side effects, which can be reduced by implementation of alternative modes of administration. In this work, glutaraldehyde cross-linked gelatin scaffolds are proposed as delivery systems of calcium alendronate monohydrate (CaALâ¢H2 O). The 3D highly porous scaffolds display a relevant interconnected porosity (>94%), independently from CaALâ¢H2 O content (0, 3, and 6 wt%). At variance, pore size varies with composition. The relative increase of the number of smaller pores on increasing BP content is in agreement with the parallel significant increase of the compressive modulus and collapse strength. The scaffolds exhibit a sustained CaALâ¢H2 O release profile, and a significant amount of the drug is retained in the scaffolds even after 14 d. In vitro tests are carried out using cocultures of osteoblast (OB) and osteoclast (OC). The evaluation of differentiation markers is performed both on the supernatants of cell culture and by means of quantitative polymerase chain reaction. The results indicate that BP containing scaffolds support osteoblast proliferation and differentiation, whereas they inhibit osteoclast viability and activity, displaying a promising beneficial role on bone repair processes.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Differentiation
/
Drug Delivery Systems
/
Cell Proliferation
/
Alginates
Limits:
Humans
Language:
En
Journal:
Macromol Biosci
Journal subject:
BIOQUIMICA
Year:
2017
Document type:
Article
Affiliation country: