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Skin regeneration with all accessory organs following ablation with irreversible electroporation.
Golberg, Alexander; Villiger, Martin; Felix Broelsch, G; Quinn, Kyle P; Albadawi, Hassan; Khan, Saiqa; Watkins, Michael T; Georgakoudi, Irene; Austen, William G; Bei, Marianna; Bouma, Brett E; Mihm, Martin C; Yarmush, Martin L.
Affiliation
  • Golberg A; Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, and the Shriners Burns Hospital, Boston, MA, 02114, USA.
  • Villiger M; Porter School of Environmental Studies, Tel Aviv University, Tel Aviv, Israel.
  • Felix Broelsch G; Wellman Center for Photomedicine and Department of Dermatology, Massachusetts General Hospital and Harvard Medical School, 50 Blossom Street, Boston, Massachusetts, 02114, USA.
  • Quinn KP; Department of Surgery, Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA.
  • Albadawi H; Department of Biomedical Engineering, Tufts University, Medford, MA, 02155, USA.
  • Khan S; Department of Biomedical Engineering, University of Arkansas, Fayetteville, AR, 72701, USA.
  • Watkins MT; Division of Vascular and Endovascular Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA.
  • Georgakoudi I; Department of Surgery, Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA.
  • Austen WG; Division of Vascular and Endovascular Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA.
  • Bei M; Department of Biomedical Engineering, Tufts University, Medford, MA, 02155, USA.
  • Bouma BE; Department of Surgery, Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA.
  • Mihm MC; Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, and the Shriners Burns Hospital, Boston, MA, 02114, USA.
  • Yarmush ML; Wellman Center for Photomedicine and Department of Dermatology, Massachusetts General Hospital and Harvard Medical School, 50 Blossom Street, Boston, Massachusetts, 02114, USA.
J Tissue Eng Regen Med ; 12(1): 98-113, 2018 01.
Article in En | MEDLINE | ID: mdl-27976527
ABSTRACT
Skin scar formation is a complex process that results in the formation of dense extracellular matrix (ECM) without normal skin appendages such as hair and glands. The absence of a scarless healing model in adult mammals prevents the development of successful therapies. We show that irreversible electroporation of skin drives its regeneration with all accessory organs in normal adult rats. Pulsed electric fields at 500 V, with 70 µs pulse duration and 1000 pulses delivered at 3 Hz, applied through two electrodes separated by 2 mm lead to massive cell death. However, the ECM architecture of the skin was preserved. Six months after the ablation, the epidermis, sebaceous glands, panniculus carnosus, hair follicles, microvasculature and arrector pili muscle were altogether re-formed in the entire ablated area. These results suggest a key role of the ECM architecture in the differentiation, migration and signalling of cells during scarless wound healing. Copyright © 2016 John Wiley & Sons, Ltd.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Regeneration / Skin / Electroporation Limits: Animals Language: En Journal: J Tissue Eng Regen Med Journal subject: BIOTECNOLOGIA / HISTOLOGIA Year: 2018 Document type: Article Affiliation country:
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Regeneration / Skin / Electroporation Limits: Animals Language: En Journal: J Tissue Eng Regen Med Journal subject: BIOTECNOLOGIA / HISTOLOGIA Year: 2018 Document type: Article Affiliation country: