Regulating chromosomal movement by the cochaperone FKB-6 ensures timely pairing and synapsis.
J Cell Biol
; 216(2): 393-408, 2017 Feb.
Article
in En
| MEDLINE
| ID: mdl-28077446
ABSTRACT
In meiotic prophase I, homologous chromosome pairing is promoted through chromosome movement mediated by nuclear envelope proteins, microtubules, and dynein. After proper homologue pairing has been established, the synaptonemal complex (SC) assembles along the paired homologues, stabilizing their interaction and allowing for crossing over to occur. Previous studies have shown that perturbing chromosome movement leads to pairing defects and SC polycomplex formation. We show that FKB-6 plays a role in SC assembly and is required for timely pairing and proper double-strand break repair kinetics. FKB-6 localizes outside the nucleus, and in its absence, the microtubule network is altered. FKB-6 is required for proper movement of dynein, increasing resting time between movements. Attenuating chromosomal movement in fkb-6 mutants partially restores the defects in synapsis, in agreement with FKB-6 acting by decreasing chromosomal movement. Therefore, we suggest that FKB-6 plays a role in regulating dynein movement by preventing excess chromosome movement, which is essential for proper SC assembly and homologous chromosome pairing.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Synaptonemal Complex
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Caenorhabditis elegans
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Molecular Chaperones
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Immunophilins
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Caenorhabditis elegans Proteins
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Chromosome Pairing
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Chromosome Positioning
Limits:
Animals
Language:
En
Journal:
J Cell Biol
Year:
2017
Document type:
Article