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Ankfy1 is dispensable for neural stem/precursor cell development.
Weng, Chao; Ding, Man; Chang, Lian-Sheng; Ren, Ming-Xin; Zhang, Hong-Feng; Lu, Zu-Neng; Fu, Hui.
Affiliation
  • Weng C; Department of Neurology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China.
  • Ding M; Department of Neurology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China.
  • Chang LS; Department of Anatomy and Embryology, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei Province, China.
  • Ren MX; Department of Anatomy and Embryology, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei Province, China.
  • Zhang HF; Department of Pathology, Central Hospital of Wuhan, Wuhan, Hubei Province, China.
  • Lu ZN; Department of Neurology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China.
  • Fu H; Department of Anatomy and Embryology, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei Province, China; Hubei-MOST KLOS & KLOBME, Wuhan, Hubei Province, China.
Neural Regen Res ; 11(11): 1804-1809, 2016 Nov.
Article in En | MEDLINE | ID: mdl-28123425
ABSTRACT
There are few studies on the membrane protein Ankfy1. We have found Ankfy1 is specifically expressed in neural stem/precursor cells during early development in mice (murine). To further explore Ankfy1 function in neural development, we developed a gene knockout mouse with a mixed Balb/C and C57/BL6 genetic background. Using immunofluorescence and in situ hybridization, neural defects were absent in mixed genetic Ankfy1 null mice during development and in adults up to 2 months old. However, Ankfy1 gene knockout mice with a pure genetic background were found to be lethal in the C57/BL6 inbred mice embryos, even after seven generations of backcrossing. Polymerase chain reaction confirmed homozygotes were unattainable as early as embryonic day 11.5. We conclude that Ankfy1 protein is dispensable in neural stem/precursor cells, but could be critical for early embryonic murine development, depending on the genetic background.
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Neural Regen Res Year: 2016 Document type: Article Affiliation country:

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Neural Regen Res Year: 2016 Document type: Article Affiliation country: