Impact of laccase on the colour stability of structured oil-in-water emulsions.
Food Res Int
; 97: 223-230, 2017 07.
Article
in En
| MEDLINE
| ID: mdl-28578045
ABSTRACT
The optical properties of food emulsions play a key role in determining their perceived quality because they are the first sensory cue that many consumers receive. The purpose of the current study was to investigate the impact of a cross-linking enzyme (laccase) on the appearance of structured oil-in-water emulsions containing a lipophilic model colorant (Nile red). A layer-by-layer electrostatic deposition approach was used to prepare oil-in-water emulsions stabilized by interfacial protein-pectin complexes under acidic conditions (pH3.5, 10mM citrate buffer). Laccase (an oxidoreductase) was then added to the system, since this enzyme is often used to covalently cross-link interfacial biopolymer layers. The optical properties of the emulsions were monitored during storage using spectral reflectance to determine the L*a*b values, while the physical properties were monitored by measuring changes in droplet surface charge and particle size distribution. No changes in the size or charge of the droplets were observed during storage, indicating that the emulsions had good physical stability. In the absence of laccase, the emulsions were stable to colour fading, but in the presence of laccase rapid colour changes occurred (red to blue to white). These results have important implications for the formation of structured food emulsions containing certain types of food dyes.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Laccase
/
Emulsions
/
Food Storage
/
Food Coloring Agents
Language:
En
Journal:
Food Res Int
Year:
2017
Document type:
Article
Affiliation country: