Immunogenicity of ORFV-based vectors expressing the rabies virus glycoprotein in livestock species.
Virology
; 511: 229-239, 2017 11.
Article
in En
| MEDLINE
| ID: mdl-28898730
ABSTRACT
The parapoxvirus Orf virus (ORFV) encodes several immunomodulatory proteins (IMPs) that modulate host-innate and pro-inflammatory responses and has been proposed as a vaccine delivery vector for use in animal species. Here we describe the construction and characterization of two recombinant ORFV vectors expressing the rabies virus (RABV) glycoprotein (G). The RABV-G gene was inserted in the ORFV024 or ORFV121 gene loci, which encode for IMPs that are unique to parapoxviruses and inhibit activation of the NF-κB signaling pathway. The immunogenicity of the resultant recombinant viruses (ORFV∆024RABV-G or ORFV∆121RABV-G, respectively) was evaluated in pigs and cattle. Immunization of the target species with ORFV∆024RABV-G and ORFV∆121RABV-G elicited robust neutralizing antibody responses against RABV. Notably, neutralizing antibody titers induced in ORFV∆121RABV-G-immunized pigs and cattle were significantly higher than those detected in ORFV∆024RABV-G-immunized animals, indicating a higher immunogenicity of ORFVΔ121-based vectors in these animal species.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Orf virus
/
Peptide Fragments
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Rabies
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Viral Proteins
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Drug Carriers
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Rabies Vaccines
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Glycoproteins
/
Genetic Vectors
Limits:
Animals
Language:
En
Journal:
Virology
Year:
2017
Document type:
Article
Affiliation country: