Paludicola psychrotolerans gen. nov., sp. nov., a novel psychrotolerant chitinolytic anaerobe of the family Ruminococcaceae.
Int J Syst Evol Microbiol
; 67(10): 4100-4103, 2017 Oct.
Article
in En
| MEDLINE
| ID: mdl-28901896
ABSTRACT
A psychrotolerant chitinolytic bacterium, designated NC1253T, was isolated from Zoige wetland on the Qinghai-Tibetan Plateau. This strain was a Gram-stain-positive, spore-forming and rod-shaped anaerobe. NC1253T grew at 4-35 °C, at pH 6.0-8.5 and could grow on chitin as the only carbon resource. Phylogenetic analysis, based on the 16S rRNA gene sequence, showed that strain NC1253T represented a novel bacterial genus within the family Ruminococcaceae. Strain NC1253T has less than 91.0â% similarity with other type strains, such as Harryflintia acetispora V20-281aT (90.9â%), Clostridium methylpentosum DSM 5476T (90.8â%), Anaerotruncus colihominis DSM 17241T (89.8â%), Eubacterium siraeum DSM 15702T (89.6â%), and Acetanaerobacterium elongatum Z7T (89.6â%). The major components of the cellular fatty acids were iso-C14ââ0, anteiso-C15ââ0, C16ââ0 and anteiso-C17ââ0. The genomic DNA G+C content was 35.4 mol%. Phenotypic, chemotaxonomic and phylogenetic characteristics allowed strain NC1253T to be clearly distinguished from genera in the family Ruminococcaceae. On the basis of polyphasic taxonomic data, the isolate is considered to represent a novel genus and novel species in the family Ruminococcaceae, for which the name Paludicola psychrotolerans gen. nov., sp. nov., is proposed. The type species is NC1253T (DSM 104738T=KCTC 15582T).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phylogeny
/
Wetlands
/
Clostridiales
Country/Region as subject:
Asia
Language:
En
Journal:
Int J Syst Evol Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2017
Document type:
Article