Determination of Arginine and Ornithine Decarboxylase Activities in Plants.
Methods Mol Biol
; 1694: 117-122, 2018.
Article
in En
| MEDLINE
| ID: mdl-29080161
ABSTRACT
In plants, putrescine is synthesized directly from the decarboxylation of ornithine and/or by the alternative arginine decarboxylase pathway. The prevalence of one or the other depends on the tissue and stress conditions. In both amino acid decarboxylation reactions, the corresponding enzymes use pyridoxal phosphate (PLP) as co-factor. PLP combines with the α-amino acid to form a Schiff base, which acts as substrate in the carboxyl group removal and CO2 formation. We describe the methodology employed for the determination of ODC and ADC activities in plant tissues by detecting the release of (C14) CO2 using (C14) labelled substrates (ornithine or arginine).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ornithine
/
Ornithine Decarboxylase
/
Arginine
/
Plants
/
Carboxy-Lyases
Type of study:
Risk_factors_studies
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2018
Document type:
Article
Affiliation country: