Sequence analysis of the thrombospondin-related adhesive protein gene and heat shock protein 70 gene of Babesia gibsoni isolated from dogs in Nanjing, China.
Infect Genet Evol
; 56: 111-116, 2017 12.
Article
in En
| MEDLINE
| ID: mdl-29154928
ABSTRACT
In this study, the thrombospondin-related adhesive protein (TRAP) gene and the heat shock protein 70 (Hsp70) gene of Babesia gibsoni isolated from the naturally infected dog in the Nanjing area were cloned and sequenced. Twenty blood samples were collected from the suspected cases of babesiosis at the animal hospital of Nanjing Agriculture University. Genomic DNA was extracted from the blood samples, and the 18S rDNAs were amplified by PCR to confirm Babesia infection. As a result, 10 cases of Babesia 18S rDNA gene amplification were positive in the 20 blood samples, confirming that the 10 cases were infected with canine Babesia. The TRAP and the Hsp70 gene fragments were amplified from all 10 positive cases. The four isolates, named NJN1, NJN2, NJN3 and NJN4, were sequenced and compared with other isolates in Asian. The similarity of TRAP and Hsp70 gene sequences among four isolates in Nanjing were above 99%. The Nanjing isolates were closely related to isolates from Taiwan and Japan. Indian isolates were different form Chinese and Japanese isolates, despite the very high similarity of the 18s rRNA genes sequence. These results suggest that the TRAP and Hsp70 genes have a reference value for the genetic diversity analysis of Babesia gibsoni.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Babesia
/
Cell Adhesion Molecules
/
HSP70 Heat-Shock Proteins
/
Thrombospondins
/
Dog Diseases
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Infect Genet Evol
Journal subject:
BIOLOGIA
/
DOENCAS TRANSMISSIVEIS
/
GENETICA
Year:
2017
Document type:
Article
Affiliation country: