Your browser doesn't support javascript.
loading
Electrochemical detection of carcinoembryonic antigen.
Gu, Xuefang; She, Zhe; Ma, Tianxiao; Tian, Shu; Kraatz, Heinz-Bernhard.
Affiliation
  • Gu X; School of Chemistry and Chemical Engineering, Nantong University, Nantong 226007, PR China; Department of Physical and Environmental Science, University of Toronto Scarborough, 1265 Military Trail, Toronto, Ontario, Canada M1C 1A4.
  • She Z; Department of Physical and Environmental Science, University of Toronto Scarborough, 1265 Military Trail, Toronto, Ontario, Canada M1C 1A4. Electronic address: zhe.she@utoronto.ca.
  • Ma T; Department of Physical and Environmental Science, University of Toronto Scarborough, 1265 Military Trail, Toronto, Ontario, Canada M1C 1A4.
  • Tian S; School of Chemistry and Chemical Engineering, Nantong University, Nantong 226007, PR China.
  • Kraatz HB; Department of Physical and Environmental Science, University of Toronto Scarborough, 1265 Military Trail, Toronto, Ontario, Canada M1C 1A4; Department of Chemistry, University of Toronto, 80. St. George Street, Toronto, Canada M5S 3H6. Electronic address: bernie.kraatz@utoronto.ca.
Biosens Bioelectron ; 102: 610-616, 2018 Apr 15.
Article in En | MEDLINE | ID: mdl-29247972
ABSTRACT
In this work, a sandwich-type electrochemical immunosensor for carcinoembryonic antigen (CEA) detection has been constructed and tested. Unlike many other sensors using external electrochemical species in the electrolyte to generate an electrochemical signal, a ferrocene derivative has been integrated into the design of the sensor to provide an internal reporting system, allowing detection of CEA in buffers and biological samples. Gold nanoparticles, which have been used to increase the conductivity of sensing surfaces, also carry immobilized secondary anti-CEA and a ferrocene derivative. The shelf life testing of the sensor shows good performance after storage for 4 weeks. The sensor has been calibrated against different concentration of the target protein using square wave voltammetry. The calibration curve has been obtained in the range of 0.05-20ngmL-1, and the detection limit for CEA is ~ 0.01ngmL-1. The capability of the immunosensor has been verified by performing detection of CEA in human serum samples.
Subject(s)
Key words
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Immunoassay / Biosensing Techniques / Carcinoembryonic Antigen / Electrochemical Techniques Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Biosens Bioelectron Journal subject: BIOTECNOLOGIA Year: 2018 Document type: Article
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Immunoassay / Biosensing Techniques / Carcinoembryonic Antigen / Electrochemical Techniques Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Biosens Bioelectron Journal subject: BIOTECNOLOGIA Year: 2018 Document type: Article