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Evaluation of the Efficiency of Lytic Mycobacteriophage D29 on the Model of M. tuberculosis-Infected Macrophage RAW 264 Cell Line.
Lapenkova, M B; Smirnova, N S; Rutkevich, P N; Vladimirsky, M A.
Affiliation
  • Lapenkova MB; Research Institute of Phthisiopulmonology, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
  • Smirnova NS; Research Institute of Phthisiopulmonology, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
  • Rutkevich PN; Research Institute of Experimental Cardiology, Russian Cardiology Research and Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia.
  • Vladimirsky MA; Research Institute of Phthisiopulmonology, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia. mvladimirskij@mail.ru.
Bull Exp Biol Med ; 164(3): 344-346, 2018 Jan.
Article in En | MEDLINE | ID: mdl-29313233
ABSTRACT
Culture of mouse macrophages (RAW 264.7 ATCC strain) in wells of a 6-well plate was infected with M. tuberculosis in proportion of 15 mycobacteria per one macrophage and then treated with a lytic strain of mycobacteriophage D29. Antibacterial efficacy of mycobacteriophages was studied using D29 phage (activity 108 plaque-forming units/ml) previously purified by ion exchange chromatography. After single and double 24-h treatment, the lysed cultures of macrophages were inoculated onto Middlebrook 7H10 agar medium. The number of mycobacterial colonies in control and test wells (at least 3 wells in each group) was 300.178±12.500 and 36.0±5.4, respectively (p<0.01).
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Lysogeny / Mycobacteriophages / Mycobacterium tuberculosis Limits: Animals Language: En Journal: Bull Exp Biol Med Year: 2018 Document type: Article Affiliation country:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Lysogeny / Mycobacteriophages / Mycobacterium tuberculosis Limits: Animals Language: En Journal: Bull Exp Biol Med Year: 2018 Document type: Article Affiliation country: