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Lymphatic exosomes promote dendritic cell migration along guidance cues.
Brown, Markus; Johnson, Louise A; Leone, Dario A; Majek, Peter; Vaahtomeri, Kari; Senfter, Daniel; Bukosza, Nora; Schachner, Helga; Asfour, Gabriele; Langer, Brigitte; Hauschild, Robert; Parapatics, Katja; Hong, Young-Kwon; Bennett, Keiryn L; Kain, Renate; Detmar, Michael; Sixt, Michael; Jackson, David G; Kerjaschki, Dontscho.
Affiliation
  • Brown M; Clinical Department of Pathology, Medical University of Vienna, Vienna, Austria.
  • Johnson LA; Institute of Science and Technology, Klosterneuburg, Austria.
  • Leone DA; Medical Research Council Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford, England, UK.
  • Majek P; Clinical Department of Pathology, Medical University of Vienna, Vienna, Austria.
  • Vaahtomeri K; CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.
  • Senfter D; Institute of Science and Technology, Klosterneuburg, Austria.
  • Bukosza N; Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Vienna, Austria.
  • Schachner H; Clinical Department of Pathology, Medical University of Vienna, Vienna, Austria.
  • Asfour G; Clinical Department of Pathology, Medical University of Vienna, Vienna, Austria.
  • Langer B; Clinical Department of Pathology, Medical University of Vienna, Vienna, Austria.
  • Hauschild R; Clinical Department of Pathology, Medical University of Vienna, Vienna, Austria.
  • Parapatics K; Institute of Science and Technology, Klosterneuburg, Austria.
  • Hong YK; CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.
  • Bennett KL; Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA.
  • Kain R; CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.
  • Detmar M; Clinical Department of Pathology, Medical University of Vienna, Vienna, Austria.
  • Sixt M; Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Zurich, Switzerland.
  • Jackson DG; Institute of Science and Technology, Klosterneuburg, Austria Michael.sixt@ist.ac.at.
  • Kerjaschki D; Medical Research Council Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford, England, UK.
J Cell Biol ; 217(6): 2205-2221, 2018 06 04.
Article in En | MEDLINE | ID: mdl-29650776
ABSTRACT
Lymphatic endothelial cells (LECs) release extracellular chemokines to guide the migration of dendritic cells. In this study, we report that LECs also release basolateral exosome-rich endothelial vesicles (EEVs) that are secreted in greater numbers in the presence of inflammatory cytokines and accumulate in the perivascular stroma of small lymphatic vessels in human chronic inflammatory diseases. Proteomic analyses of EEV fractions identified >1,700 cargo proteins and revealed a dominant motility-promoting protein signature. In vitro and ex vivo EEV fractions augmented cellular protrusion formation in a CX3CL1/fractalkine-dependent fashion and enhanced the directional migratory response of human dendritic cells along guidance cues. We conclude that perilymphatic LEC exosomes enhance exploratory behavior and thus promote directional migration of CX3CR1-expressing cells in complex tissue environments.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Dendritic Cells / Cell Movement / Lymphatic Vessels / Exosomes Type of study: Guideline / Prognostic_studies Limits: Animals / Humans / Male Language: En Journal: J Cell Biol Year: 2018 Document type: Article Affiliation country:
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Dendritic Cells / Cell Movement / Lymphatic Vessels / Exosomes Type of study: Guideline / Prognostic_studies Limits: Animals / Humans / Male Language: En Journal: J Cell Biol Year: 2018 Document type: Article Affiliation country: