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Reconstitution of full-length P450BM3 with an artificial metal complex by utilising the transpeptidase Sortase A.
Omura, Keita; Aiba, Yuichiro; Onoda, Hiroki; Stanfield, Joshua Kyle; Ariyasu, Shinya; Sugimoto, Hiroshi; Shiro, Yoshitsugu; Shoji, Osami; Watanabe, Yoshihito.
Affiliation
  • Omura K; Department of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-0802, Japan. shoji.osami@a.mbox.nagoya-u.ac.jp.
Chem Commun (Camb) ; 54(57): 7892-7895, 2018 Jul 12.
Article in En | MEDLINE | ID: mdl-29845154
ABSTRACT
Haem substitution is an effective approach to tweak the function of haemoproteins. Herein, we report a facile haem substitution method for self-sufficient cytochrome P450BM3 (CYP102A1) from Bacillus megaterium utilising the transpeptidase Sortase A from Staphylococcus aureus. We successfully constructed Mn-substituted BM3 and investigated its catalytic activity.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Cysteine Endopeptidases / Aminoacyltransferases / Cytochrome P-450 Enzyme System / Coordination Complexes Language: En Journal: Chem Commun (Camb) Journal subject: QUIMICA Year: 2018 Document type: Article Affiliation country:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Cysteine Endopeptidases / Aminoacyltransferases / Cytochrome P-450 Enzyme System / Coordination Complexes Language: En Journal: Chem Commun (Camb) Journal subject: QUIMICA Year: 2018 Document type: Article Affiliation country: