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The Generation and Use of Allergen-Specific TCR Transgenic Animals.
Vanheerswynghels, Manon; Toussaint, Wendy; Schuijs, Martijn; Vanhoutte, Leen; Killeen, Nigel; Hammad, Hamida; Lambrecht, Bart N.
Affiliation
  • Vanheerswynghels M; VIB-UGent Center for Inflammation Research, Ghent, Belgium.
  • Toussaint W; Department of Respiratory Medicine, Gent University, Ghent, Belgium.
  • Schuijs M; VIB-UGent Center for Inflammation Research, Ghent, Belgium.
  • Vanhoutte L; Department of Respiratory Medicine, Gent University, Ghent, Belgium.
  • Killeen N; VIB-UGent Center for Inflammation Research, Ghent, Belgium.
  • Hammad H; Department of Respiratory Medicine, Gent University, Ghent, Belgium.
  • Lambrecht BN; VIB-UGent Center for Inflammation Research, Ghent, Belgium.
Methods Mol Biol ; 1799: 183-210, 2018.
Article in En | MEDLINE | ID: mdl-29956153
ABSTRACT
The generation of allergen-specific TCR transgenic animals allows for the characterization of allergen-specific T-cell responses in vivo and in vitro and is a powerful tool to study adaptive immunity to allergens. Here we describe an approach starting from the isolation of antigen-specific T-cell hybridomas and using PCR, flow cytometric, and co-culture methods to obtain antigen-specific MHC class II-restricted CD4+ TCR transgenic mice on the Rag2-/- background.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Allergens / Receptors, Antigen, T-Cell / T-Lymphocyte Subsets / T-Cell Antigen Receptor Specificity Limits: Animals Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2018 Document type: Article Affiliation country:
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Allergens / Receptors, Antigen, T-Cell / T-Lymphocyte Subsets / T-Cell Antigen Receptor Specificity Limits: Animals Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2018 Document type: Article Affiliation country: