Transfection Mediated by DEAE-Dextran.
Cold Spring Harb Protoc
; 2018(7)2018 07 02.
Article
in En
| MEDLINE
| ID: mdl-29967275
ABSTRACT
Here, we describe two variations on the classical DEAE-dextran transfection procedure. The first involves a brief exposure of cells to a high concentration of DEAE-dextran and yields higher transfection frequencies but elevated cellular toxicity. The second involves a longer exposure of cells to a lower concentration of DEAE-dextran, which produces lower transfection frequencies but increased cell survival.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transfection
/
DEAE-Dextran
Limits:
Animals
Language:
En
Journal:
Cold Spring Harb Protoc
Year:
2018
Document type:
Article