Endoscopic molecular imaging of early gastric cancer using fluorescently labeled human H-ferritin nanoparticle.
Nanomedicine
; 14(7): 2259-2270, 2018 10.
Article
in En
| MEDLINE
| ID: mdl-30056091
ABSTRACT
Optical imaging technologies improve clinical diagnostic accuracy of early gastric cancer (EGC). However, there was a lack of imaging agents exhibiting molecular specificity for EGCs. Here, we employed the dye labeled human heavy-chain ferritin (HFn) as imaging nanoprobe, which recognizes tumor biomarker transferrin receptor 1 (TfR1), to enable specific EGC imaging using confocal laser endomicroscopy (CLE). TfR1 expression was initially examined in vitro in gastric tumor cells and in vivo through whole-body fluorescence and CLE imaging in tumor-bearing mice. Subsequently, dye labeled HFn was topically applied to resected human tissues for EGC detection. CLE analysis of TfR1-targeted fluorescence imaging allowed distinction of neoplastic from non-neoplastic tissues (Pâ¯<â¯0.0001), and TfR1 expression level was found to correlate with EGC differentiation degrees (Pâ¯<â¯0.0001). Notably, the CLE evaluation correlated well with the immunohistochemical findings (κ-coefficient 0.8023). Our HFn-nanoprobe-based CLE increases the accuracy of EGC detection and enables visualization of tumor margins and endoscopic resection.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Apoferritins
/
Stomach Neoplasms
/
Receptors, Transferrin
/
Antigens, CD
/
Endoscopy
/
Nanoparticles
/
Molecular Imaging
/
Fluorescent Dyes
Type of study:
Prognostic_studies
Limits:
Aged
/
Aged80
/
Animals
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Nanomedicine
Journal subject:
BIOTECNOLOGIA
Year:
2018
Document type:
Article