Microglia increases the proliferation of retinal precursor cells during postnatal development.
Mol Vis
; 24: 536-545, 2018.
Article
in En
| MEDLINE
| ID: mdl-30090016
ABSTRACT
Purpose:
In mice, retinal development continues throughout the postnatal stage accompanied by the proliferation of retinal precursor cells. Previous reports showed that during the postnatal stage microglia increase from postnatal day 0 (P0) to P7. However, how microglia are associated with retinal development remains unknown.Methods:
The involvement of microglia in retinal development was investigated by two approaches, microglial activation and loss, using lipopolysaccharide (LPS) and PLX3397 (pexidartinib), respectively.Results:
LPS injection at 1 mg/kg, intraperitoneally (i.p.) in the neonatal mice increased the number of retinal microglia at P7. 5-Bromo-2´-deoxyuridine (BrdU)-positive proliferative cells were increased by LPS treatment compared to the control group. The proliferative cells were mainly colocalized with paired box 6 (Pax6), a marker of retinal precursor cells. However, the depletion of microglia by treatment with PLX3397 decreased the BrdU-positive proliferative cells. Moreover, progranulin deficiency decreased the number of microglia and retinal precursor cells.Conclusions:
These findings indicated that microglia regulate the proliferation of immature retinal cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Retina
/
Stem Cells
/
Microglia
/
Gene Expression Regulation, Developmental
/
Intercellular Signaling Peptides and Proteins
/
PAX6 Transcription Factor
Language:
En
Journal:
Mol Vis
Journal subject:
BIOLOGIA MOLECULAR
/
OFTALMOLOGIA
Year:
2018
Document type:
Article
Affiliation country: