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Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification.
Vaidyanathan, Sriram; Azizian, Krist T; Haque, A K M Ashiqul; Henderson, Jordana M; Hendel, Ayal; Shore, Sabrina; Antony, Justin S; Hogrefe, Richard I; Kormann, Michael S D; Porteus, Matthew H; McCaffrey, Anton P.
Affiliation
  • Vaidyanathan S; Department of Pediatrics, Stanford University, Stanford, CA, USA.
  • Azizian KT; TriLink BioTechnologies, San Diego, CA, USA.
  • Haque AKMA; Department of Pediatrics I, Pediatric Infectiology and Immunology, Translational Genomics and Gene Therapy in Pediatrics, University of Tuebingen, Tuebingen, Germany.
  • Henderson JM; TriLink BioTechnologies, San Diego, CA, USA.
  • Hendel A; The Mina and Everard Goodman Faculty of Life Sciences and Advanced Materials and Nanotechnology Institute, Bar-Ilan University, Ramat-Gan 52900, Israel.
  • Shore S; TriLink BioTechnologies, San Diego, CA, USA.
  • Antony JS; Department of Pediatrics I, Pediatric Infectiology and Immunology, Translational Genomics and Gene Therapy in Pediatrics, University of Tuebingen, Tuebingen, Germany.
  • Hogrefe RI; TriLink BioTechnologies, San Diego, CA, USA.
  • Kormann MSD; Department of Pediatrics I, Pediatric Infectiology and Immunology, Translational Genomics and Gene Therapy in Pediatrics, University of Tuebingen, Tuebingen, Germany.
  • Porteus MH; Department of Pediatrics, Stanford University, Stanford, CA, USA.
  • McCaffrey AP; TriLink BioTechnologies, San Diego, CA, USA. Electronic address: antonmccaffrey@gmail.com.
Mol Ther Nucleic Acids ; 12: 530-542, 2018 Sep 07.
Article in En | MEDLINE | ID: mdl-30195789
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Mol Ther Nucleic Acids Year: 2018 Document type: Article Affiliation country:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Mol Ther Nucleic Acids Year: 2018 Document type: Article Affiliation country: