Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines.
Virology
; 525: 1-9, 2018 12.
Article
in En
| MEDLINE
| ID: mdl-30205273
ABSTRACT
Investigating type I feline coronaviruses (FCoVs) in tissue culture is critical for understanding the basic virology, pathogenesis, and virus-host interactome of these important veterinary pathogens. This has been a perennial challenge as type I FCoV strains do not easily adapt to cell culture. Here we characterize replication kinetics and plaque formation of a model type I strain FIPV Black in Fcwf-4 cells established at Cornell University (Fcwf-4 CU). We determined that maximum virus titers (>107 pfu/mL) were recoverable from infected Fcwf-4 CU cell-free supernatant at 20â¯h post-infection. Type I FIPV Black and both biotypes of type II FCoV formed uniform and enumerable plaques on Fcwf-4 CU cells. Therefore, these cells were employable in a standardized plaque assay. Finally, we determined that the Fcwf-4 CU cells were morphologically distinct from feline bone marrow-derived macrophages and were less sensitive to exogenous type I interferon than were Fcwf-4 cells purchased from ATCC.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Viral Plaque Assay
/
Virus Cultivation
/
Virus Replication
/
Coronavirus, Feline
Limits:
Animals
Language:
En
Journal:
Virology
Year:
2018
Document type:
Article
Affiliation country: