A case of laboratory cross-contamination of Mycobacterium tuberculosis identified using comparative genomics.
Int J Tuberc Lung Dis
; 22(10): 1239-1242, 2018 10 01.
Article
in En
| MEDLINE
| ID: mdl-30236195
ABSTRACT
BACKGROUND:
Two false-positive tuberculosis (TB) cases in Yamagata Prefecture, Japan, 2016.OBJECTIVE:
To report the effectiveness of comparative genomics of Mycobacterium tuberculosis for identification of cross-contamination cases.DESIGN:
Case report of laboratory cross-contamination.RESULTS:
Beginning with detection of an identical genotype in two M. tuberculosis strains using variable number of tandem repeat typing, we suspected M. tuberculosis cross-contamination of specimens collected in a mycobacteriology laboratory based on epidemiological investigations. This suspicion was confirmed using comparative genomics of the two M. tuberculosis strains and a strain from an epidemiologically unrelated specimen from the same batch as the two strains in the mycobacteriology laboratory. All strains had an identical genomic sequence with no single nucleotide variants.CONCLUSION:
Comparative genomics, which offers the highest discrimination power, is a potent tool for identifying laboratory cross-contamination using epidemiological investigations.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tuberculosis
/
Genomics
/
False Positive Reactions
/
Mycobacterium tuberculosis
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Humans
Country/Region as subject:
Asia
Language:
En
Journal:
Int J Tuberc Lung Dis
Year:
2018
Document type:
Article