Pedobacter chitinilyticus sp. nov., a chitin-degrading bacterium isolated from wheat leaf tissue.
Int J Syst Evol Microbiol
; 68(12): 3713-3719, 2018 Dec.
Article
in En
| MEDLINE
| ID: mdl-30307389
ABSTRACT
A bacterium, designated strain CM134L-2T, was isolated from a chitin-enriched wheat leaf microbiome in Chengdu, Sichuan province, China. It was Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow in colour. Strain CM134L-2T grew at 4-35 °C, at pH 6.0-9.0 and could use chitin as the only carbon resource. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CM134L-2T was most closely related to Pedobacter nanyangensis Q-4T (97.7â%) and Pedobacter zeaxanthinifaciens TDMA-5T (97.4â%). Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2T with these two type strains were 26.8â and 20.8â%, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2â%; these values are lower than the proposed and generally accepted species boundaries of 70â% for dDDH and 95-96â% for ANI, which suggests strain CM134L-2T represents a novel species. The genomic DNA G+C content of strain CM134L-2T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15ââ0, and C16ââ1ω7c/C16ââ1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2T to the genus Pedobacter. Overall, strain CM134L-2T belongs to the genus Pedobacter, but can be classified as a novel species, for which the name Pedobacter chitinilyticus sp. nov. is proposed. The type strain is CM134L-2T (=CGMCC 1.16520T=KCTC 62643T).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phylogeny
/
Triticum
/
Pedobacter
Country/Region as subject:
Asia
Language:
En
Journal:
Int J Syst Evol Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2018
Document type:
Article