[Experimental Study on Paeoniflorin Inhibiting mTOR Signaling Pathway in Adjuvant Arthritis Rats].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 49(4): 535-539, 2018 Jul.
Article
in Zh
| MEDLINE
| ID: mdl-30378305
ABSTRACT
OBJECTIVE:
To study the effect of paeoniflorin (PF) on mTOR signal in synovial fibroblast-like synoviocytes (FLS) in rats with adjuvant arthritis.METHODS:
AA model rats were prepared by complete Freun's adjuvant injection in foot-plantar, the PF was injected to rats in AA + PF 100 µg / mL group, AA + PF 200 µg / mL group and AA + PF 400 µg / mL group by the tail vein injection at the dose of 0.1 mL/200 g body mass, and the effects of three doses of PF on arthritis scores in AA rats were studied. The modeling rats and control rats were sacrificed at 28 d after modeling, then the synovium was separeated from rat articular, the FLS were cultured. The effect of PF on the expression of mTOR and MMP3 in AA FLS was detected by the real time qPCR. The effect on the cytokine IL-1, IL-6 was detected by ELISA, and the Western blot was used to investigate the role of PF in the mTOR phosphorylation. Furthermore, FLS were transfected with mTOR vectors, and the effect of mTOR overexpression on the PF roles was detected by real time qPCR and ELISA.RESULTS:
The tail vein injection of PF can significantly reduce the AA rat arthritis score. Compared with AA group, the expression of mTOR in AA+PF 1 µg/mL, AA+PF 2 µg/mL, AA+PF 4 µg/mL was significantly decreased at 48 h after dosing. Compared with AA group, the relative expression of p-mTOR protein in PF 2 µg/mL group was also decreased. Compared with AA group at 48 h after dosing, the levels of IL-1, IL-6 and MMP3 in AA+PF 1 µg/mL, AA+PF 2 µg/mL, AA+PF 4 µg/ mL were significantly decreased, respectively. Compared with PF 2 µg/mL group, the relative expression of IL-1, IL-6 and MMP3 in PF 2 µg/mL+mTOR vectors was increased.CONCLUSION:
PF can significantly inhibit the pathology of AA rats, and its mechanism may be related to the inhibition of mTOR signal in FLS of AA rats.Key words
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arthritis, Experimental
/
Signal Transduction
/
Monoterpenes
/
TOR Serine-Threonine Kinases
/
Fibroblasts
/
Glucosides
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Sichuan Da Xue Xue Bao Yi Xue Ban
Year:
2018
Document type:
Article
Affiliation country:
Publication country:
CHINA
/
CN
/
REPUBLIC OF CHINA