Diverse preparation methods for small intestinal submucosa (SIS): Decellularization, components, and structure.
J Biomed Mater Res A
; 107(3): 689-697, 2019 03.
Article
in En
| MEDLINE
| ID: mdl-30468308
ABSTRACT
The native extracellular matrix (ECM) biomaterial derived from small intestinal submucosa (SIS) is widely applied in tissue engineering for tissue repair and regeneration. SIS ECM is obtained through physical and chemical methods to remove the intrinsic cells, which would otherwise cause adverse immune reactions when the SIS ECM is implanted into the host body. Several research teams have reported diverse SIS decellularization methods. However, there was no consensus on the criteria to be used for the decellularization methods for SIS and further research on the mechanism action of SIS is needed for comprehensive detection of the biological composition. In this present study, we used three reported methods to prepare SIS and compared their effects on decellularization and the remaining biological components, microstructure and cytocompatibility. SIS prepared by the three kinds of decellularization methods all achieved the recommended criteria, had good biocompatibility and retained most active components. Nevertheless, regardless of which decellularization method was used, the microstructure and bioactive components of the prepared SIS were damaged in varying degrees. We recommend that researchers need to select a decellularization method that would be appropriate to use according to their research purposes. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A 107A 689-697, 2019.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bone Marrow Cells
/
Tissue Engineering
/
Extracellular Matrix
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Tissue Scaffolds
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Mesenchymal Stem Cells
/
Intestinal Mucosa
/
Intestine, Small
Limits:
Animals
Language:
En
Journal:
J Biomed Mater Res A
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2019
Document type:
Article
Affiliation country: